Hotspot

trnK-UUU-psbA

LSC · 75888-76181 · 294 bp

Back to Macrotyloma

Pi 0.0068

MarkerSeek score 59.20

Primer available yes

Label trnK-UUU-psbA

Feature Metadata

label_name: trnK-UUU-psbA
feature_type: igs
region: LSC
length_bp: 294
variable_sites: 3
pi: 0.0068
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-psbA_p1	AAAATGCAAACACGGCTTGG	AAACGACCCCATAGGCTTTC	45.0	50.0	58.4	57.9	458-464 bp	1.0	77.2
trnK-UUU-psbA_p2	AAAATGCAAACACGGCTTGG	GAAACGACCCCATAGGCTTT	45.0	50.0	58.4	57.9	459-465 bp	1.0	77.2
trnK-UUU-psbA_p3	CTTCATCCGACTAGTTCCGG	AAACGACCCCATAGGCTTTC	55.0	50.0	57.5	57.9	379-385 bp	1.0	76.9
trnK-UUU-psbA_p4	CTTCATCCGACTAGTTCCGG	GAAACGACCCCATAGGCTTT	55.0	50.0	57.5	57.9	380-386 bp	1.0	76.9
trnK-UUU-psbA_p5	AAAATGCAAACACGGCTTGG	CCAGTTACAGAAACGACCCC	45.0	55.0	58.4	58.2	468-474 bp	1.0	76.6