Hotspot

trnF-GAA-ndhJ

LSC · 64591-65381 · 791 bp

Back to Macrotyloma

Pi 0.0119

MarkerSeek score 59.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 14
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATAGTCATCCGCGTCTGAGA	50.0	50.0	58.1	58.0	924-936 bp	1.0	81.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.1	57.9	884-896 bp	1.0	81.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GATAGTCATCCGCGTCTGAG	50.0	55.0	58.1	57.7	925-937 bp	1.0	79.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGATAGTCATCCGCGTCTGA	50.0	50.0	58.1	58.3	926-938 bp	1.0	79.1
trnF-GAA-ndhJ_p5	AATCCTCGTGTCACCAGTTC	ATAGTCATCCGCGTCTGAGA	50.0	50.0	57.5	58.0	927-939 bp	1.0	78.2