Hotspot

trnL-UAA-trnF-GAA

LSC · 64122-64517 · 396 bp

Back to Macrotyloma

Pi 0.0109

MarkerSeek score 59.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 396
variable_sites: 6
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	274-475 bp	1.0	77.1
trnL-UAA-trnF-GAA_p2	TTCAAGTCCCTCTATCCCCA	CTTGTGCCAGGAACCAGATT	50.0	50.0	57.0	58.1	292-493 bp	1.0	76.8
trnL-UAA-trnF-GAA_p3	TTCAAGTCCCTCTATCCCCA	AATCTTGTGCCAGGAACCAG	50.0	50.0	57.0	58.1	295-496 bp	1.0	76.7
trnL-UAA-trnF-GAA_p4	TTCAAGTCCCTCTATCCCCA	GAATCTTGTGCCAGGAACCA	50.0	50.0	57.0	58.1	296-497 bp	1.0	76.7
trnL-UAA-trnF-GAA_p5	TTCAAGTCCCTCTATCCCCA	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.0	58.1	289-490 bp	1.0	76.7