Hotspot

psaA-ycf3

LSC · 58548-59281 · 734 bp

Back to Macrotyloma

Pi 0.00727

MarkerSeek score 63.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 8
pi: 0.00727
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AATGACCTGGTTTTGCCCAT	TTGGTTGAAGATCACAGGGC	45.0	50.0	58.0	58.1	858-865 bp	1.0	79.9
psaA-ycf3_p2	ATGACCTGGTTTTGCCCATT	TTGGTTGAAGATCACAGGGC	45.0	50.0	58.0	58.1	857-864 bp	1.0	79.9
psaA-ycf3_p3	TGGTTTTGCCCATTCCTCAA	TTGGTTGAAGATCACAGGGC	45.0	50.0	57.9	58.1	851-858 bp	1.0	79.4
psaA-ycf3_p4	AATGACCTGGTTTTGCCCAT	TGGTTTAATCAAGCCGCTGA	45.0	45.0	58.0	57.8	932-939 bp	1.0	79.0
psaA-ycf3_p5	ATGACCTGGTTTTGCCCATT	TGGTTTAATCAAGCCGCTGA	45.0	45.0	58.0	57.8	931-938 bp	1.0	79.0