Hotspot

psbC-trnS-UGA

LSC · 51854-52054 · 201 bp

Back to Macrotyloma

Pi 0.0299

MarkerSeek score 71.50

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 201
variable_sites: 9
pi: 0.0299
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TCGCGATTTTGAACCTGTTC	GAGAGATGGCTGAGTGGTTG	45.0	55.0	57.0	58.0	336 bp	1.0	74.3
psbC-trnS-UGA_p2	CGCGATTTTGAACCTGTTCT	GAGAGATGGCTGAGTGGTTG	45.0	55.0	57.0	58.0	335 bp	1.0	74.2
psbC-trnS-UGA_p3	TCGCGATTTTGAACCTGTTC	ATAGCCCCGGTCTTGAAAAC	45.0	50.0	57.0	57.9	316 bp	1.0	73.6
psbC-trnS-UGA_p4	TCGCGATTTTGAACCTGTTC	GCTTTTGGAGAGATGGCTGA	45.0	50.0	57.0	57.9	343 bp	1.0	73.4
psbC-trnS-UGA_p5	TCGCGATTTTGAACCTGTTC	AGCTTTTGGAGAGATGGCTG	45.0	50.0	57.0	57.9	344 bp	1.0	73.4