Hotspot

atpH-atpI

LSC · 29892-30944 · 1053 bp

Back to Macrotyloma

Pi 0.00443

MarkerSeek score 63.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1053
variable_sites: 7
pi: 0.00443
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAGCAGCAATAACAGAAGCG	TCACAAGCGGTATTCAAGCT	50.0	45.0	57.8	57.5	1155-1173 bp	1.0	76.7
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	TCACAAGCGGTATTCAAGCT	50.0	45.0	57.8	57.5	1162-1180 bp	1.0	76.3
atpH-atpI_p3	CAATCCCCTCTACAGCTTGC	TCACAAGCGGTATTCAAGCT	55.0	45.0	58.3	57.5	1233-1251 bp	1.0	75.3
atpH-atpI_p4	GCAATCCCCTCTACAGCTTG	TCACAAGCGGTATTCAAGCT	55.0	45.0	58.3	57.5	1234-1252 bp	1.0	75.3
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCT	TCACAAGCGGTATTCAAGCT	50.0	45.0	57.6	57.5	1185-1203 bp	1.0	74.6