Hotspot

rpl33-psaJ

LSC · 13237-13745 · 509 bp

Back to Macrotyloma

Pi 0.00917

MarkerSeek score 38.90

Primer available yes

Label rpl33-psaJ

Feature Metadata

label_name: rpl33-psaJ
feature_type: igs
region: LSC
length_bp: 509
variable_sites: 7
pi: 0.00917
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-psaJ_p1	CGGATATCTTTACCCTTGGCC	CATATCTTTCCGTGGCACCA	52.4	50.0	58.2	58.0	643-651 bp	1.0	71.8
rpl33-psaJ_p2	CGGATATCTTTACCCTTGGCC	ACATATCTTTCCGTGGCACC	52.4	50.0	58.2	58.0	644-652 bp	1.0	71.8
rpl33-psaJ_p3	CGGATATCTTTACCCTTGGCC	GCTTTGGCGGGTCTATTGAT	52.4	50.0	58.2	58.3	599-607 bp	1.0	69.7
rpl33-psaJ_p4	CGGATATCTTTACCCTTGGCC	ATATCTTTCCGTGGCACCAG	52.4	50.0	58.2	57.7	642-650 bp	1.0	69.4
rpl33-psaJ_p5	CGGATATCTTTACCCTTGGCC	TTTATGGTTCGGGGCTTTGG	52.4	50.0	58.2	58.4	612-620 bp	1.0	68.6