Hotspot

ycf1

SSC · 98818-104061 · 5244 bp

Back to Macrosolen

Pi 0.0715

MarkerSeek score 78.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5244
variable_sites: 712
pi: 0.0715
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTGCGCATTCAGACTAGG	GGGGAATGGAAGCTGATCTG	50.0	55.0	58.0	58.0	634-664 bp	0.5	63.3
ycf1_p2	AGTTGCGCATTCAGACTAGG	AGTTTGGGGAATGGAAGCTG	50.0	50.0	58.0	58.1	639-669 bp	0.5	62.9
ycf1_p3	AGTTGCGCATTCAGACTAGG	CAGTTTGGGGAATGGAAGCT	50.0	50.0	58.0	58.1	640-670 bp	0.5	62.8
ycf1_p4	AGTTGCGCATTCAGACTAGG	GTTTGGGGAATGGAAGCTGA	50.0	50.0	58.0	58.1	638-668 bp	0.5	62.8
ycf1_p5	AGTTGCGCATTCAGACTAGG	GTCTTTCACATCCCAACGGT	50.0	50.0	58.0	58.1	528-558 bp	0.5	62.5