Hotspot

ccsA-psaC

SSC · 97156-98010 · 855 bp

Back to Macrosolen

Pi 0.103
MarkerSeek score 74.90
Primer available yes
Label ccsA-psaC

Feature Metadata

label_name
ccsA-psaC
feature_type
igs
region
SSC
length_bp
855
variable_sites
116
pi
0.103
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.65
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-psaC_p1 AGGTTTTGCTACTTCGGGTC CATGTATCGGGTGCACTCAA 50.0 50.0 57.8 58.0 1466-1695 bp 0.75 78.8
ccsA-psaC_p2 AGGTTTTGCTACTTCGGGTC TGTATCGGGTGCACTCAATG 50.0 50.0 57.8 58.0 1464-1693 bp 0.75 78.8
ccsA-psaC_p3 GGTTTTGCTACTTCGGGTCT CATGTATCGGGTGCACTCAA 50.0 50.0 57.8 58.0 1465-1694 bp 0.75 78.8
ccsA-psaC_p4 GGTTTTGCTACTTCGGGTCT TGTATCGGGTGCACTCAATG 50.0 50.0 57.8 58.0 1463-1692 bp 0.75 78.8
ccsA-psaC_p5 GGTTTTGCTACTTCGGGTCT GTATCGGGTGCACTCAATGT 50.0 50.0 57.8 58.0 1462-1691 bp 0.75 78.8