Hotspot

rpl36-rps8

LSC · 66090-66346 · 257 bp

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Pi 0.168

MarkerSeek score 77.60

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 79
pi: 0.168
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CAGCAAATGGTGTCCCTCTT	CGAGTTTTTGGTGGGATTGC	50.0	50.0	58.1	57.9	1324-1344 bp	0.75	71.1
rpl36-rps8_p2	TGTCCGAGTGGCTACTGTTA	CGAGTTTTTGGTGGGATTGC	50.0	50.0	58.1	57.9	1628-1648 bp	0.75	70.9
rpl36-rps8_p3	CAGCAAATGGTGTCCCTCTT	AAGGAACGGTTCGAATAGCC	50.0	50.0	58.1	58.0	1037-1048 bp	0.5	60.2
rpl36-rps8_p4	TGTCCGAGTGGCTACTGTTA	AAGGAACGGTTCGAATAGCC	50.0	50.0	58.1	58.0	1341-1352 bp	0.5	60.2
rpl36-rps8_p5	CATGACTTCCGCTCCTTTCA	AAGGAACGGTTCGAATAGCC	50.0	50.0	57.9	58.0	1105-1116 bp	0.5	60.1