Hotspot

clpP-psbB

LSC · 58567-58985 · 419 bp

Back to Macrosolen

Pi 0.0569

MarkerSeek score 82.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 419
variable_sites: 46
pi: 0.0569
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	AGGGTACTCTTGGAACACCA	CACGATACCAAGGTAAACCCA	50.0	47.6	57.9	57.7	469-556 bp	1.0	78.4
clpP-psbB_p2	AGGGTACTCTTGGAACACCA	ACACGATACCAAGGTAAACCC	50.0	47.6	57.9	57.7	470-557 bp	1.0	78.4
clpP-psbB_p3	AGGGTACTCTTGGAACACCA	ACGATACCAAGGTAAACCCA	50.0	45.0	57.9	56.2	468-555 bp	1.0	74.3
clpP-psbB_p4	AGGGTACTCTTGGAACACCA	CACGATACCAAGGTAAACCCAT	50.0	45.5	57.9	58.1	469-556 bp	1.0	72.1
clpP-psbB_p5	AGGGTACTCTTGGAACACCA	CACGATACCAAGGTAAACCC	50.0	50.0	57.9	55.8	469-556 bp	1.0	71.8