Hotspot

rpl20-rps12

LSC · 55647-56335 · 689 bp

Back to Macrosolen

Pi 0.0535

MarkerSeek score 84.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 70
pi: 0.0535
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGAATGAGCTCCTCGAAAGC	TATAAAGCAACGGGGCCATC	50.0	50.0	58.0	58.0	1501-1646 bp	1.0	90.1
rpl20-rps12_p2	TGAATGAGCTCCTCGAAAGC	TTCTGAAATTGCGCGAAACC	50.0	45.0	58.0	58.0	1181-1327 bp	1.0	89.7
rpl20-rps12_p3	GAAGGATCCACAAACGACGA	TATAAAGCAACGGGGCCATC	50.0	50.0	57.9	58.0	1604-1749 bp	1.0	89.5
rpl20-rps12_p4	TGAATGAGCTCCTCGAAAGC	TGTCACAAAATCCCCTGCTC	50.0	50.0	58.0	58.1	840-892 bp	0.75	71.4
rpl20-rps12_p5	TGAATGAGCTCCTCGAAAGC	AAGATGCCTGTACGGTGTTC	50.0	50.0	58.0	57.9	1342-1347 bp	0.5	60.1