Hotspot

rbcL-accD

LSC · 45959-46575 · 617 bp

Back to Macrosolen

Pi 0.1

MarkerSeek score 83.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 617
variable_sites: 116
pi: 0.1
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGAACCTTAGGACACCCT	CCGTGCTCAAACTCTTCCTT	55.0	50.0	58.0	58.1	870-948 bp	1.0	90.7
rbcL-accD_p2	GCCTTACGTATGTCTGGTGG	CCGTGCTCAAACTCTTCCTT	55.0	50.0	58.1	58.1	1132-1210 bp	1.0	90.6
rbcL-accD_p3	GCCGTAGCTAATCGAGTAGC	CCGTGCTCAAACTCTTCCTT	55.0	50.0	58.0	58.1	832-910 bp	0.75	80.8
rbcL-accD_p4	AGTTCGGTGGAGGAACCTTA	CCGTGCTCAAACTCTTCCTT	50.0	50.0	58.0	58.1	879-957 bp	0.75	75.3
rbcL-accD_p5	GGGGATTCACGGCAAATACT	CCGTGCTCAAACTCTTCCTT	50.0	50.0	57.9	58.1	1266-1344 bp	0.75	73.3