Hotspot

atpB-rbcL

LSC · 43918-44530 · 613 bp

Back to Macrosolen

Pi 0.0636
MarkerSeek score 84.30
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
613
variable_sites
75
pi
0.0636
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 TTGCCTGGGGGAAAAACTAC GTAAGTCCATCGGTCCACAC 50.0 55.0 58.0 58.0 953-975 bp 0.75 72.5
atpB-rbcL_p2 TTGCCTGGGGGAAAAACTAC CGATCAAGGCTGGTAAGTCC 50.0 55.0 58.0 58.1 965-987 bp 0.75 72.0
atpB-rbcL_p3 TTGCCTGGGGGAAAAACTAC ACACTCATAAACCGCTCGAC 50.0 50.0 58.0 58.0 1305-1327 bp 0.75 71.9
atpB-rbcL_p4 TTGCCTGGGGGAAAAACTAC AGATCCTCCAGACGTAGAGC 50.0 55.0 58.0 58.0 1142-1164 bp 0.75 71.9
atpB-rbcL_p5 TTGCCTGGGGGAAAAACTAC CGGTCTCTCCAACGCATAAA 50.0 50.0 58.0 58.0 1379-1401 bp 0.75 71.8