Hotspot

psaA-ycf3

LSC · 36287-36835 · 549 bp

Back to Macrosolen

Pi 0.0692

MarkerSeek score 82.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 549
variable_sites: 68
pi: 0.0692
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	ATCTGGGCCTTTCGCTATTG	TGGTTGAAGATTACGAGGCG	50.0	50.0	58.0	58.0	656-757 bp	0.75	77.4
psaA-ycf3_p2	ATCTGGGCCTTTCGCTATTG	GGTTGAAGATTACGAGGCGT	50.0	50.0	58.0	58.0	655-756 bp	0.75	77.4
psaA-ycf3_p3	GGCACTAGGTCCAATGTGAG	TGGTTGAAGATTACGAGGCG	55.0	50.0	58.0	58.0	869-970 bp	0.75	76.6
psaA-ycf3_p4	TAATGGAACCAACCCGCAAA	TGGTTGAAGATTACGAGGCG	45.0	50.0	58.0	58.0	1066-1167 bp	0.75	75.6
psaA-ycf3_p5	TAATGGAACCAACCCGCAAA	GGTTGAAGATTACGAGGCGT	45.0	50.0	58.0	58.0	1065-1166 bp	0.75	75.6