Hotspot

trnD-GUC-trnY-GUA

LSC · 25795-26214 · 420 bp

Back to Macrosolen

Pi 0.0774

MarkerSeek score 81.70

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 420
variable_sites: 61
pi: 0.0774
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GTGCTCTAACCAGTTGAACT	TCTACGCTGGTTCAAATCCG	45.0	50.0	55.3	58.0	446-479 bp	1.0	70.5
trnD-GUC-trnY-GUA_p2	TGCTCTAACCAGTTGAACTACA	TCTACGCTGGTTCAAATCCG	40.9	50.0	57.1	58.0	445-478 bp	1.0	68.4
trnD-GUC-trnY-GUA_p3	ACCAGTTGAACTACAATCCC	TCTACGCTGGTTCAAATCCG	45.0	50.0	55.0	58.0	438-471 bp	1.0	67.6
trnD-GUC-trnY-GUA_p4	AACCAGTTGAACTACAATCCC	TCTACGCTGGTTCAAATCCG	42.9	50.0	55.8	58.0	439-472 bp	1.0	66.2
trnD-GUC-trnY-GUA_p5	GTGCTCTAACCAGTTGAACTAC	TCTACGCTGGTTCAAATCCG	45.5	50.0	56.7	58.0	446-479 bp	1.0	65.9