Hotspot

petN-psbM

LSC · 24196-24762 · 567 bp

Back to Macrosolen

Pi 0.075

MarkerSeek score 83.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 567
variable_sites: 79
pi: 0.075
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GATTCGGGAAAGGGGGATTC	TGATTGTACAAGGACAGCGG	55.0	50.0	58.0	57.9	1571-1617 bp	0.5	60.8
petN-psbM_p2	GATTCGGGAAAGGGGGATTC	GATTGTACAAGGACAGCGGT	55.0	50.0	58.0	57.9	1570-1616 bp	0.5	60.8
petN-psbM_p3	TTTCCCCAGTTCAAATCCGG	TGATTGTACAAGGACAGCGG	50.0	50.0	58.1	57.9	1424-1472 bp	0.5	60.2
petN-psbM_p4	GCATAACTGACGAAGGGAGG	TGATTGTACAAGGACAGCGG	55.0	50.0	58.1	57.9	1694 bp	0.25	48.8
petN-psbM_p5	GCATAACTGACGAAGGGAGG	GATTGTACAAGGACAGCGGT	55.0	50.0	58.1	57.9	1693 bp	0.25	48.8