Hotspot

rpoB-trnC-GCA

LSC · 23062-23729 · 668 bp

Back to Macrosolen

Pi 0.118

MarkerSeek score 78.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 133
pi: 0.118
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCCATCCCAGAGC	CTTACCACTCGGCCATGC	55.0	61.1	58.0	58.2	366-900 bp	1.0	74.3
rpoB-trnC-GCA_p2	CCTCATTTCCATCCCAGAGC	CCTTACCACTCGGCCATG	55.0	61.1	58.0	57.5	367-901 bp	1.0	71.5
rpoB-trnC-GCA_p3	CTCATTTCCATCCCAGAGCA	CTTACCACTCGGCCATGC	50.0	61.1	57.3	58.2	365-899 bp	1.0	68.7
rpoB-trnC-GCA_p4	CTCATTTCCATCCCAGAGCA	CCTTACCACTCGGCCATG	50.0	61.1	57.3	57.5	366-900 bp	1.0	65.9
rpoB-trnC-GCA_p5	CTCATTTCCATCCCAGAGCAT	CTTACCACTCGGCCATGC	47.6	61.1	57.8	58.2	365-899 bp	1.0	64.7