Hotspot

trnS-GCU-trnR-UCU

LSC · 6294-6689 · 396 bp

Back to Macrosolen

Pi 0.0932

MarkerSeek score 78.10

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 396
variable_sites: 62
pi: 0.0932
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ATTAGCAATCCGCCGCTTTA	ACCTCTGTCCTATCCATTAGACA	45.0	43.5	58.0	57.9	467-611 bp	1.0	66.5
trnS-GCU-trnR-UCU_p2	CTTTAGCCCACTCAGCCATC	ACCTCTGTCCTATCCATTAGACA	55.0	43.5	58.3	57.9	452-596 bp	1.0	64.2
trnS-GCU-trnR-UCU_p3	TTAGCAATCCGCCGCTTTAG	ACCTCTGTCCTATCCATTAGACA	50.0	43.5	58.7	57.9	466-610 bp	1.0	61.2
trnS-GCU-trnR-UCU_p4	TTTAGCCCACTCAGCCATCT	ACCTCTGTCCTATCCATTAGACA	50.0	43.5	58.7	57.9	451-595 bp	1.0	61.2
trnS-GCU-trnR-UCU_p5	ATTAGCAATCCGCCGCTTTA	CCTCTGTCCTATCCATTAGACA	45.0	45.5	58.0	56.2	466-610 bp	1.0	61.0