Hotspot

matK-trnK-UUU

LSC · 3449-4157 · 709 bp

Back to Macrosolen

Pi 0.0503

MarkerSeek score 81.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 65
pi: 0.0503
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGTCCTGTTTCCGAGCTAGA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.8	58.2	779-789 bp	0.75	70.4
matK-trnK-UUU_p2	AGTCCTGTTTCCGAGCTAGA	ACCGGTCGTCTGATTCTTTG	50.0	50.0	57.8	57.9	1122-1334 bp	0.5	58.5
matK-trnK-UUU_p3	AGTCCTGTTTCCGAGCTAGA	CAACCGGTCGTCTGATTCTT	50.0	50.0	57.8	57.9	1124-1336 bp	0.5	58.5
matK-trnK-UUU_p4	AGTCCTGTTTCCGAGCTAGA	CCATAGGAATCAACCGGTCG	50.0	55.0	57.8	58.1	1134-1346 bp	0.5	58.1
matK-trnK-UUU_p5	AGTCCTGTTTCCGAGCTAGA	TAGGAATCAACCGGTCGTCT	50.0	50.0	57.8	58.2	1131-1343 bp	0.5	57.9