Hotspot

ndhF

SSC · 112682-114910 · 2229 bp

Back to Macrolenes

Pi 0.0135

MarkerSeek score 54.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 30
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCCAAAATGTCAATCCAGAGT	TCGAAGGAGTTGAAAAAGAA	38.1	35.0	55.1	52.7	2306-2312 bp	1.0	41.2
ndhF_p2	CATCCAAAATGTCAATCCAGAGT	TCGAAGGAGTTGAAAAAGAA	39.1	35.0	57.1	52.7	2308-2314 bp	1.0	41.2
ndhF_p3	TCCAACAAGGAAGAATTGTCT	TCGAAGGAGTTGAAAAAGAA	38.1	35.0	55.0	52.7	2363-2369 bp	1.0	41.2
ndhF_p4	TCATCCAAAATGTCAATCCAGAG	TCGAAGGAGTTGAAAAAGAA	39.1	35.0	56.8	52.7	2309-2315 bp	1.0	41.2
ndhF_p5	TCCAACAAGGAAGAATTGTCTT	TCGAAGGAGTTGAAAAAGAA	36.4	35.0	55.8	52.7	2363-2369 bp	1.0	41.2