Hotspot

petG-trnW-CCA

LSC · 67588-67724 · 137 bp

Back to Macrolenes

Pi 0.101

MarkerSeek score 79.90

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 13
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACGTGGTGATCAGTTAGACC	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.0	57.9	201 bp	1.0	79.4
petG-trnW-CCA_p2	GACGTGGTGATCAGTTAGACC	CAAAACCCGATGTCGTAGGT	52.4	50.0	58.1	57.9	202 bp	1.0	78.6
petG-trnW-CCA_p3	ACGTGGTGATCAGTTAGACC	CCCGATGTCGTAGGTTCAAA	50.0	50.0	57.0	57.6	196 bp	1.0	77.3
petG-trnW-CCA_p4	ACGTGGTGATCAGTTAGACC	CTCCAAAACCCGATGTCGTA	50.0	50.0	57.0	57.6	204 bp	1.0	77.0
petG-trnW-CCA_p5	ACGTGGTGATCAGTTAGACC	TCCAAAACCCGATGTCGTAG	50.0	50.0	57.0	57.6	203 bp	1.0	77.0