Hotspot

petL-petG

LSC · 67281-67473 · 193 bp

Back to Macrolenes

Pi 0.0311
MarkerSeek score 66.60
Primer available yes
Label petL-petG

Feature Metadata

label_name
petL-petG
feature_type
igs
region
LSC
length_bp
193
variable_sites
6
pi
0.0311
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-petG_p1 TCATTTAGCCCCTTCATGCT GGTCTAACTGATCACCACGT 45.0 50.0 56.9 57.0 413 bp 1.0 63.9
petL-petG_p2 TCATTTAGCCCCTTCATGCT GGTCTAACTGATCACCACGTC 45.0 52.4 56.9 58.1 413 bp 1.0 63.1
petL-petG_p3 TCATTTAGCCCCTTCATGCTT GGTCTAACTGATCACCACGT 42.9 50.0 57.6 57.0 413 bp 1.0 61.8
petL-petG_p4 TTCATTTAGCCCCTTCATGCT GGTCTAACTGATCACCACGT 42.9 50.0 57.6 57.0 414 bp 1.0 61.8
petL-petG_p5 TCATTTAGCCCCTTCATGCT AGGTCTAACTGATCACCACG 45.0 50.0 56.9 56.7 414 bp 1.0 61.5