Hotspot

rbcL-accD

LSC · 58181-58835 · 655 bp

Back to Macrolenes

Pi 0.0107

MarkerSeek score 65.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 7
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	TTTCCCGCTAGACAACATCG	50.0	50.0	57.9	58.0	755-757 bp	1.0	80.1
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	TTTCCCGCTAGACAACATCG	55.0	50.0	57.7	58.0	771-773 bp	1.0	78.7
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	CCCGCTAGACAACATCGAAT	50.0	50.0	57.9	57.8	752-754 bp	1.0	78.4
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	ACATCGAATTAAACCGCCGT	50.0	45.0	57.9	58.3	741-743 bp	1.0	78.0
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	CGAATTAAACCGCCGTTGTT	50.0	45.0	57.9	57.7	737-739 bp	1.0	77.6