Hotspot

psbZ-trnG-UCC

LSC · 37572-38096 · 525 bp

Back to Macrolenes

Pi 0.00962

MarkerSeek score 63.60

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 525
variable_sites: 5
pi: 0.00962
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GACGGCTGGTCAAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	671-677 bp	1.0	78.8
psbZ-trnG-UCC_p2	ACGGCTGGTCAAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.7	57.8	670-676 bp	1.0	77.1
psbZ-trnG-UCC_p3	CGTTGTGTTTGCTTCTCCTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.6	57.8	690-696 bp	1.0	76.7
psbZ-trnG-UCC_p4	TGTGTTTGCTTCTCCTGACG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	687-693 bp	1.0	76.5
psbZ-trnG-UCC_p5	CCGTTGTGTTTGCTTCTCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.7	57.8	691-697 bp	1.0	74.3