Hotspot

atpF-atpH

LSC · 13426-14064 · 639 bp

Back to Macrolenes

Pi 0.0192

MarkerSeek score 66.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 12
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGCCCAAGGAAAGGAAAGAA	GGATTGGTTGTCGCTTTAGC	50.0	50.0	58.0	57.5	724-729 bp	1.0	78.5
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GGATTGGTTGTCGCTTTAGC	50.0	50.0	57.9	57.5	756-761 bp	1.0	77.5
atpF-atpH_p3	GGCCCAAGGAAAGGAAAGAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	789-794 bp	1.0	77.2
atpF-atpH_p4	GGCCCAAGGAAAGGAAAGAA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	790-795 bp	1.0	77.0
atpF-atpH_p5	GGCCCAAGGAAAGGAAAGAA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	791-796 bp	1.0	77.0