Hotspot

ycf46

LSC · 25302-26777 · 1476 bp

Back to Macrocystis

Pi 0.00203

MarkerSeek score 59.90

Primer available yes

Label ycf46

Feature Metadata

label_name: ycf46
feature_type: gene
region: LSC
length_bp: 1476
variable_sites: 3
pi: 0.00203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf46_p1	GCAATAGCTAACAATATTATTATAGCT	GGTTGCAAAGTAATAAACAACCG	25.9	39.1	54.2	57.2	1702 bp	1.0	40.1
ycf46_p2	GCAATAGCTAACAATATTATTATAGC	GGTTGCAAAGTAATAAACAACCG	26.9	39.1	52.9	57.2	1702 bp	1.0	40.1
ycf46_p3	GCAATAGCTAACAATATTATTATAGCT	AAACAACCGTAGACGAAAAA	25.9	35.0	54.2	53.7	1688 bp	1.0	40.1
ycf46_p4	GCAATAGCTAACAATATTATTATAGCT	GTTGCAAAGTAATAAACAACCGT	25.9	34.8	54.2	56.5	1701 bp	1.0	40.1
ycf46_p5	GCAATAGCTAACAATATTATTATAGC	AAACAACCGTAGACGAAAAA	26.9	35.0	52.9	53.7	1688 bp	1.0	40.1