Hotspot

ycf42

LSC · 41912-42472 · 561 bp

Back to Macrocystis

Pi 0.00535

MarkerSeek score 61.00

Primer available yes

Label ycf42

Feature Metadata

label_name: ycf42
feature_type: gene
region: LSC
length_bp: 561
variable_sites: 3
pi: 0.00535
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf42_p1	AAGTCCATTACGGTCATGCC	GCTACCCGGAACAAACTTGA	50.0	50.0	58.0	58.1	1250 bp	1.0	79.0
ycf42_p2	GTCCATTACGGTCATGCCTT	GCTACCCGGAACAAACTTGA	50.0	50.0	58.0	58.1	1248 bp	1.0	79.0
ycf42_p3	TTTTTCCAACGAGCAGGGAA	GCTACCCGGAACAAACTTGA	45.0	50.0	57.9	58.1	1212 bp	1.0	78.8
ycf42_p4	TGAGAGTTGGTGCCAAGATG	GCTACCCGGAACAAACTTGA	50.0	50.0	57.8	58.1	1594 bp	1.0	77.7
ycf42_p5	GTCCATTACGGTCATGCCTT	TTGCTACCCGGAACAAACTT	50.0	45.0	58.0	57.6	1250 bp	1.0	77.1