Hotspot

rpoC2

LSC · 10651-14487 · 3837 bp

Back to Macrocystis

Pi 0.00313

MarkerSeek score 43.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 3837
variable_sites: 12
pi: 0.00313
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TCTGTATCATTTGATTGAAGTGA	TCGCCCTATAACTCGGTATGA	30.4	47.6	53.8	57.9	201 bp	1.0	41.2
rpoC2_p2	TCTGTATCATTTGATTGAAGTGAA	TCGCCCTATAACTCGGTATGA	29.2	47.6	54.5	57.9	201 bp	1.0	41.2
rpoC2_p3	TTCTGTATCATTTGATTGAAGTGA	TCGCCCTATAACTCGGTATGA	29.2	47.6	54.5	57.9	202 bp	1.0	41.2
rpoC2_p4	TTCTGTATCATTTGATTGAAGTGAA	TCGCCCTATAACTCGGTATGA	28.0	47.6	55.3	57.9	202 bp	1.0	41.2
rpoC2_p5	TCTGTATCATTTGATTGAAGTGA	CGCCCTATAACTCGGTATGA	30.4	50.0	53.8	56.3	200 bp	1.0	41.2