Hotspot

psbJ-psbL

LSC · 72987-73053 · 67 bp

Back to Macrocystis

Pi 0.0149

MarkerSeek score 61.10

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 1
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	GCCACCAACTAATGCGACTA	CGTACGTCTTTATACTGGGGG	50.0	52.4	58.0	57.6	193 bp	1.0	69.4
psbJ-psbL_p2	CCTAGGCCACCAACTAATGC	CGTACGTCTTTATACTGGGGG	55.0	52.4	58.3	57.6	198 bp	1.0	67.0
psbJ-psbL_p3	AACTCCTAGGCCACCAACTA	CGTACGTCTTTATACTGGGGG	50.0	52.4	57.7	57.6	202 bp	1.0	66.8
psbJ-psbL_p4	ACTCCTAGGCCACCAACTAA	CGTACGTCTTTATACTGGGGG	50.0	52.4	57.7	57.6	201 bp	1.0	66.8
psbJ-psbL_p5	TAACTCCTAGGCCACCAACT	CGTACGTCTTTATACTGGGGG	50.0	52.4	57.7	57.6	203 bp	1.0	66.8