Hotspot

trnE-psaA

LSC · 47795-48028 · 234 bp

Back to Macrocystis

Pi 0.0214

MarkerSeek score 64.80

Primer available yes

Label trnE-psaA

Feature Metadata

label_name: trnE-psaA
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 5
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-psaA_p1	TAAGCCCCCATCGTCTAGAG	ACGTGAAAAATGACCTGGCT	55.0	45.0	58.0	58.0	412 bp	1.0	80.8
trnE-psaA_p2	CATCTCCTTTTCACGGAGGG	ACGTGAAAAATGACCTGGCT	55.0	45.0	58.0	58.0	384 bp	1.0	80.7
trnE-psaA_p3	ATCTCCTTTTCACGGAGGGA	ACGTGAAAAATGACCTGGCT	50.0	45.0	58.0	58.0	383 bp	1.0	80.5
trnE-psaA_p4	TAAGCCCCCATCGTCTAGAG	TTGGCTGCTTGCTTATTGGA	55.0	45.0	58.0	58.1	336 bp	1.0	80.5
trnE-psaA_p5	CATCTCCTTTTCACGGAGGG	TTGGCTGCTTGCTTATTGGA	55.0	45.0	58.0	58.1	308 bp	1.0	80.4