Hotspot

trnP-chlB

LSC · 21203-21275 · 73 bp

Back to Macrocystis

Pi 0.0137

MarkerSeek score 60.40

Primer available yes

Label trnP-chlB

Feature Metadata

label_name: trnP-chlB
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 1
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-chlB_p1	CCAAAGCAGATACGCTACCA	CGAAACACCGAAAAGTTTGCT	50.0	42.9	57.7	58.2	197 bp	1.0	68.3
trnP-chlB_p2	CCAAAGCAGATACGCTACCA	CGAAACACCGAAAAGTTTGC	50.0	45.0	57.7	56.8	197 bp	1.0	68.0
trnP-chlB_p3	CCCAAAGCAGATACGCTACC	CGAAACACCGAAAAGTTTGCT	55.0	42.9	58.4	58.2	198 bp	1.0	67.4
trnP-chlB_p4	CCCAAAGCAGATACGCTACC	CGAAACACCGAAAAGTTTGC	55.0	45.0	58.4	56.8	198 bp	1.0	67.1
trnP-chlB_p5	CCAAAGCAGATACGCTACCA	ACACCGAAAAGTTTGCTCAAG	50.0	42.9	57.7	57.6	193 bp	1.0	66.6