Hotspot

rps16

LSC · 4942-6178 · 1237 bp

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Pi 0.0162

MarkerSeek score 56.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1237
variable_sites: 20
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAATTACACAAAGATAGGTACT	TGTATCGGTTCAGTCAATGGT	30.4	42.9	52.6	57.0	1350-1356 bp	1.0	41.7
rps16_p2	AAGAATTACACAAAGATAGGTACT	TGTATCGGTTCAGTCAATGGT	29.2	42.9	53.4	57.0	1351-1357 bp	1.0	41.7
rps16_p3	AGAATTACACAAAGATAGGTACT	TGTATCGGTTCAGTCAATGGTT	30.4	40.9	52.6	57.7	1350-1356 bp	1.0	41.7
rps16_p4	AAAGAATTACACAAAGATAGGTACT	TGTATCGGTTCAGTCAATGGT	28.0	42.9	54.2	57.0	1352-1358 bp	1.0	41.7
rps16_p5	AAGAATTACACAAAGATAGGTACT	TGTATCGGTTCAGTCAATGGTT	29.2	40.9	53.4	57.7	1351-1357 bp	1.0	41.7