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psbN-psbH

LSC · 72925-73044 · 120 bp

Back to Macodes

Pi 0.0435

MarkerSeek score 67.30

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 5
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	TCCTTTGCCATCTTTTGCCT	CGGTTGCGAGGTCTGAATAA	45.0	50.0	58.0	58.0	1280-1299 bp	1.0	82.1
psbN-psbH_p2	TCCTTTGCCATCTTTTGCCT	CACACGCTCATATTCCGGAA	45.0	50.0	58.0	58.1	1105-1124 bp	1.0	81.9
psbN-psbH_p3	TCAACTAGTCCCCGTGTTCT	CGGTTGCGAGGTCTGAATAA	50.0	50.0	58.0	58.0	799-805 bp	1.0	81.5
psbN-psbH_p4	TCAACTAGTCCCCGTGTTCT	CACACGCTCATATTCCGGAA	50.0	50.0	58.0	58.1	624-630 bp	1.0	81.5
psbN-psbH_p5	GAAGATGGCGACTAGGGTTG	CGGTTGCGAGGTCTGAATAA	55.0	50.0	58.1	58.0	699 bp	0.5	59.5