Hotspot

psbB-psbT

LSC · 72287-72620 · 334 bp

Back to Macodes

Pi 0.0218

MarkerSeek score 57.90

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 334
variable_sites: 7
pi: 0.0218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCCAACTACAAAGAGACAAGCA	GAACTTTAGGCGGTTCTCGA	40.9	50.0	57.6	57.6	443-450 bp	1.0	61.4
psbB-psbT_p2	TCCAACTACAAAGAGACAAGCA	TGGAACTTTAGGCGGTTCTC	40.9	50.0	57.6	57.5	445-452 bp	1.0	60.5
psbB-psbT_p3	TCCAACTACAAAGAGACAAGCA	GGAACTTTAGGCGGTTCTCG	40.9	55.0	57.6	58.6	444-451 bp	1.0	59.1
psbB-psbT_p4	TCCAACTACAAAGAGACAAGCA	AGTTGGAACTTTAGGCGGTT	40.9	45.0	57.6	57.3	448-455 bp	1.0	59.0
psbB-psbT_p5	CCAACTACAAAGAGACAAGCAG	GAACTTTAGGCGGTTCTCGA	45.5	50.0	57.3	57.6	442-449 bp	1.0	58.8