Hotspot

rpl33-rps18

LSC · 65931-66089 · 159 bp

Back to Macodes

Pi 0.0323

MarkerSeek score 73.40

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 5
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACACCCAATCG	TAATCGATTCGATCCCCCGA	50.0	50.0	56.9	58.1	331-335 bp	1.0	72.8
rpl33-rps18_p2	CGACACAATACACCCAATCG	GGGGTAAACGCCTACGAAAA	50.0	50.0	56.9	58.2	303-307 bp	1.0	72.1
rpl33-rps18_p3	CGACACAATACACCCAATCGA	TAATCGATTCGATCCCCCGA	47.6	50.0	58.4	58.1	331-335 bp	1.0	70.4
rpl33-rps18_p4	TCGACACAATACACCCAATCG	TAATCGATTCGATCCCCCGA	47.6	50.0	58.4	58.1	332-336 bp	1.0	70.4
rpl33-rps18_p5	CGACACAATACACCCAATCG	CCGATCCAATTGGGGGTAAA	50.0	50.0	56.9	57.6	315-319 bp	1.0	70.0