Hotspot

psaJ-rpl33

LSC · 65138-65729 · 592 bp

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Pi 0.0139

MarkerSeek score 69.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 8
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TGGTTTGGGTCTTTAGCAGG	CACCCTTTTGAACACAACAGG	50.0	47.6	57.7	57.8	733-743 bp	1.0	71.2
psaJ-rpl33_p2	GGTTTGGGTCTTTAGCAGGT	CACCCTTTTGAACACAACAGG	50.0	47.6	57.7	57.8	732-742 bp	1.0	71.2
psaJ-rpl33_p3	TGGTTTGGGTCTTTAGCAGG	ACCCTTTTGAACACAACAGGT	50.0	42.9	57.7	58.2	732-742 bp	1.0	71.1
psaJ-rpl33_p4	GGTTTGGGTCTTTAGCAGGT	ACCCTTTTGAACACAACAGGT	50.0	42.9	57.7	58.2	731-741 bp	1.0	71.0
psaJ-rpl33_p5	GGCACCTGTACTAACCACTC	CACCCTTTTGAACACAACAGG	55.0	47.6	57.6	57.8	755-765 bp	1.0	70.4