Hotspot

rbcL-accD

LSC · 55144-55939 · 796 bp

Back to Macodes

Pi 0.0165

MarkerSeek score 55.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 13
pi: 0.0165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CCAGTGGATAAGCTAGATAAA	ACAACATCGAATTGAACCAACA	38.1	36.4	52.1	57.1	851-854 bp	1.0	41.9
rbcL-accD_p2	CCAGTGGATAAGCTAGATAAAT	ACAACATCGAATTGAACCAACA	36.4	36.4	52.8	57.1	851-854 bp	1.0	41.9
rbcL-accD_p3	CCAGTGGATAAGCTAGATAAA	AACAACATCGAATTGAACCAACA	38.1	34.8	52.1	57.7	852-855 bp	1.0	41.9
rbcL-accD_p4	CCAGTGGATAAGCTAGATAAA	ACAACATCGAATTGAACCAACAT	38.1	34.8	52.1	57.5	851-854 bp	1.0	41.9
rbcL-accD_p5	CCAGTGGATAAGCTAGATAAAT	AACAACATCGAATTGAACCAACA	36.4	34.8	52.8	57.7	852-855 bp	1.0	41.9