Hotspot

trnS-GCU-trnG-GCC

LSC · 7857-8357 · 501 bp

Back to Macodes

Pi 0.0265
MarkerSeek score 67.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
501
variable_sites
13
pi
0.0265
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 560-570 bp 1.0 54.9
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 559-569 bp 1.0 54.9
trnS-GCU-trnG-GCC_p3 GTTATCAGGGGAGTCGGAGA ACGAATCACACTTTTACCACT 55.0 38.1 57.9 55.5 627-637 bp 1.0 54.1
trnS-GCU-trnG-GCC_p4 AGTTATCAGGGGAGTCGGAG ACGAATCACACTTTTACCACT 55.0 38.1 57.9 55.5 628-638 bp 1.0 54.0
trnS-GCU-trnG-GCC_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 562-572 bp 1.0 53.9