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trnK-UUU-rps16

LSC · 4319-4941 · 623 bp

Back to Macodes

Pi 0.0129

MarkerSeek score 53.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 623
variable_sites: 8
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1298-1303 bp	1.0	80.1
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1297-1302 bp	1.0	80.1
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	889-893 bp	1.0	79.4
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	888-892 bp	1.0	79.4
trnK-UUU-rps16_p5	AGGATCAGTCGCGGTCTTAT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.3	58.0	1383-1388 bp	1.0	78.9