Hotspot

ccsA

SSC · 121061-122032 · 972 bp

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Pi 0.00412

MarkerSeek score 47.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 4
pi: 0.00412
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AACAAGCCGCTATGGTGAAA	AATCCAGCGAGAACCCTTTG	45.0	50.0	58.1	58.2	1312 bp	1.0	78.6
ccsA_p2	AACAAGCCGCTATGGTGAAA	CCAGCGAGAACCCTTTGAAT	45.0	50.0	58.1	58.2	1309 bp	1.0	78.6
ccsA_p3	AACAAGCCGCTATGGTGAAA	AAAGGGTTCTCGCTGGATTG	45.0	50.0	58.1	58.2	1265 bp	1.0	78.3
ccsA_p4	AACAAGCCGCTATGGTGAAA	ATTCAAAGGGTTCTCGCTGG	45.0	50.0	58.1	58.2	1269 bp	1.0	78.3
ccsA_p5	AACAAGCCGCTATGGTGAAA	CAAAGGGTTCTCGCTGGATT	45.0	50.0	58.1	58.2	1266 bp	1.0	78.3