Hotspot

ccsA-ndhD

SSC · 122033-122303 · 271 bp

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Pi 0.0332

MarkerSeek score 67.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 271
variable_sites: 9
pi: 0.0332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCCGCAATTGTAGCCTCG	TCCCGATTTCGTTTTATCACT	50.0	38.1	58.7	55.3	457 bp	1.0	46.6
ccsA-ndhD_p2	CCGCAATTGTAGCCTCGAT	TCCCGATTTCGTTTTATCACT	52.6	38.1	58.0	55.3	454 bp	1.0	44.2
ccsA-ndhD_p3	GCAATTGTAGCCTCGATGGG	TCCCGATTTCGTTTTATCACT	55.0	38.1	59.1	55.3	452 bp	1.0	43.8
ccsA-ndhD_p4	ATTCCGCAATTGTAGCCTCG	TCGATCTCTATTCTTTTACCCGT	50.0	39.1	58.7	56.9	498 bp	1.0	43.0
ccsA-ndhD_p5	ATTCCGCAATTGTAGCCTCG	ATCCCGATTTCGTTTTATCACT	50.0	36.4	58.7	55.9	458 bp	1.0	43.0