Hotspot

rpl32-trnL-UAG

SSC · 118972-120862 · 1891 bp

Back to Maclura

Pi 0.00858
MarkerSeek score 51.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1891
variable_sites
16
pi
0.00858
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGAAGGGGTACAATCTCGAA ATCCGCATAAAAGAGCTGCA 45.0 45.0 55.9 58.0 2839-2847 bp 1.0 64.0
rpl32-trnL-UAG_p2 TGAAGGGGTACAATCTCGAA TCCGCATAAAAGAGCTGCAT 45.0 45.0 55.9 58.0 2838-2846 bp 1.0 64.0
rpl32-trnL-UAG_p3 ATTGGGAAGCATTGAAAGCT ATCCGCATAAAAGAGCTGCA 40.0 45.0 55.9 58.0 2641-2649 bp 1.0 64.0
rpl32-trnL-UAG_p4 ATTGGGAAGCATTGAAAGCT TCCGCATAAAAGAGCTGCAT 40.0 45.0 55.9 58.0 2640-2648 bp 1.0 64.0
rpl32-trnL-UAG_p5 TGAAGGGGTACAATCTCGAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 55.9 57.9 2214-2222 bp 1.0 63.6