Hotspot

petA-psbJ

LSC · 67412-68524 · 1113 bp

Back to Maclura

Pi 0.00453

MarkerSeek score 62.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1113
variable_sites: 5
pi: 0.00453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACAAGGCCTTTTGCTCTTCT	CTTGTGATCGGTTTACTCGGT	45.0	47.6	57.6	58.0	1278-1285 bp	1.0	69.5
petA-psbJ_p2	AGGCCTTTTGCTCTTCTTGG	CTTGTGATCGGTTTACTCGGT	50.0	47.6	58.4	58.0	1275-1282 bp	1.0	69.5
petA-psbJ_p3	TCCATTACGTGTACAAGGCC	CTTGTGATCGGTTTACTCGGT	50.0	47.6	57.6	58.0	1290-1297 bp	1.0	69.4
petA-psbJ_p4	CCATTACGTGTACAAGGCCT	CTTGTGATCGGTTTACTCGGT	50.0	47.6	57.6	58.0	1289-1296 bp	1.0	69.3
petA-psbJ_p5	ACAAGGCCTTTTGCTCTTCT	TTGTGATCGGTTTACTCGGT	45.0	45.0	57.6	56.9	1277-1284 bp	1.0	69.0