Hotspot

psaI-ycf4

LSC · 63607-64049 · 443 bp

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Pi 0.0092

MarkerSeek score 62.50

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 4
pi: 0.0092
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	TCGAGACCCCGCTATAAGTT	45.0	50.0	58.0	57.9	547-555 bp	1.0	81.0
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	TTCGAGACCCCGCTATAAGT	45.0	50.0	58.0	57.9	548-556 bp	1.0	81.0
psaI-ycf4_p3	GGGCTTAGTATTTCCGGCAA	TCGAGACCCCGCTATAAGTT	50.0	50.0	58.0	57.9	556-564 bp	1.0	80.8
psaI-ycf4_p4	GGGCTTAGTATTTCCGGCAA	TTCGAGACCCCGCTATAAGT	50.0	50.0	58.0	57.9	557-565 bp	1.0	80.8
psaI-ycf4_p5	CGGCAATTGCAATGGTTTCT	TCGAGACCCCGCTATAAGTT	45.0	50.0	57.9	57.9	542-550 bp	1.0	80.6