Hotspot

psbM-trnD-GUC

LSC · 31814-32320 · 507 bp

Back to Maclura

Pi 0.0118

MarkerSeek score 56.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 507
variable_sites: 6
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	618-620 bp	1.0	59.1
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	619-621 bp	1.0	55.0
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	45.0	52.6	55.8	57.8	617-619 bp	1.0	53.1
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	AGTTCAATTGGTCAGAGCACC	45.0	47.6	55.8	58.5	620-622 bp	1.0	51.0
psbM-trnD-GUC_p5	TAGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	42.9	50.0	55.7	58.5	619-621 bp	1.0	50.7