Hotspot

rpoB-trnC-GCA

LSC · 27938-29144 · 1207 bp

Back to Maclura

Pi 0.0117
MarkerSeek score 66.40
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1207
variable_sites
14
pi
0.0117
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCTTTCCTCATTTCCACCCC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.1 58.1 1287-1296 bp 1.0 79.8
rpoB-trnC-GCA_p2 CTTTCCTCATTTCCACCCCC CCGGATTTGAACTGGGGAAA 55.0 50.0 58.1 58.1 1286-1295 bp 1.0 79.8
rpoB-trnC-GCA_p3 CCTTTCCTCATTTCCACCCC TTGTTGATTAGGCGACACCC 55.0 50.0 58.1 58.2 1305-1314 bp 1.0 78.9
rpoB-trnC-GCA_p4 CTTTCCTCATTTCCACCCCC TTGTTGATTAGGCGACACCC 55.0 50.0 58.1 58.2 1304-1313 bp 1.0 78.9
rpoB-trnC-GCA_p5 CTTTCCTCATTTCCACCCCC AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.1 58.4 1268-1277 bp 1.0 77.6