Hotspot

atpF-atpH

LSC · 13595-14082 · 488 bp

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Pi 0.0102

MarkerSeek score 62.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 5
pi: 0.0102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GATGGCCAATAACCCAAGGA	GAGGCAGAGGGGAAAATACG	50.0	55.0	57.5	58.0	652-674 bp	1.0	76.6
atpF-atpH_p2	GATGGCCAATAACCCAAGGA	GCAGAGGGGAAAATACGAGG	50.0	55.0	57.5	58.0	649-671 bp	1.0	76.6
atpF-atpH_p3	GATGGCCAATAACCCAAGGA	GGCAGAGGGGAAAATACGAG	50.0	55.0	57.5	58.0	650-672 bp	1.0	76.6
atpF-atpH_p4	GGATGGCCAATAACCCAAGG	GCAGAGGGGAAAATACGAGG	55.0	55.0	58.6	58.0	650-672 bp	1.0	75.5
atpF-atpH_p5	GGATGGCCAATAACCCAAGG	GGCAGAGGGGAAAATACGAG	55.0	55.0	58.6	58.0	651-673 bp	1.0	75.5