Hotspot

ycf1

SSC · 130540-136101 · 5562 bp

Back to Macleaya

Pi 0.00833

MarkerSeek score 46.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5562
variable_sites: 46
pi: 0.00833
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCGCAGTTCCTTATGTTGT	TGTCGAGAAAGAGGAAATGCT	45.0	42.9	57.4	57.3	240 bp	1.0	63.7
ycf1_p2	ACCGCAGTTCCTTATGTTGT	TCGAGAAAGAGGAAATGCTGA	45.0	42.9	57.4	57.0	238 bp	1.0	61.5
ycf1_p3	TGACCGCAGTTCCTTATGTT	TGTCGAGAAAGAGGAAATGCT	45.0	42.9	57.1	57.3	242 bp	1.0	61.4
ycf1_p4	TTGACCGCAGTTCCTTATGT	TGTCGAGAAAGAGGAAATGCT	45.0	42.9	57.1	57.3	243 bp	1.0	61.4
ycf1_p5	ACCGCAGTTCCTTATGTTGT	GTCGAGAAAGAGGAAATGCTG	45.0	47.6	57.4	57.0	239 bp	1.0	61.1