Hotspot

rpl22

IRa · 161802-162332 · 531 bp

Back to Macleaya

Pi 0.391

MarkerSeek score 68.00

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: IRa
length_bp: 531
variable_sites: 52
pi: 0.391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.25
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TGGGTAGAGCCGGATCTAAG	TTCCTCGTGGTTTACCCTCT	55.0	50.0	58.0	58.0	1409 bp	0.5	59.8
rpl22_p2	CCACAATGATCGGCCATACA	TTCCTCGTGGTTTACCCTCT	50.0	50.0	58.0	58.0	1003 bp	0.5	59.6
rpl22_p3	TGGGTAGAGCCGGATCTAAG	CCATTATTCCTCGTCCGCAA	55.0	50.0	58.0	58.1	1795 bp	0.5	59.6
rpl22_p4	TGGGTAGAGCCGGATCTAAG	CCTCGTCCGCAAATATCCAA	55.0	50.0	58.0	58.1	1787 bp	0.5	59.6
rpl22_p5	TCCCGGGCATCTACCATTAT	TTCCTCGTGGTTTACCCTCT	50.0	50.0	58.0	58.0	1025 bp	0.5	59.5